Objective To investigate the real-time effect of JIN’s three-needle combined with mirror therapy on upper limb function in patients with stroke and upper extremity dysfunction. Methods A total of 11 patients with stroke and upper extremity dysfunction were selected. Acupuncture treatment was performed in the following acupoints: three needles in the temple of opposite side of the affected limb

three needles in the shoulder and hand of the upper limbs of the affected side

Renzhong（ GV26） and Baihui（ GV20）. While needling retention

mirror rehabilitation was performed for 30 min. Resting state functional magnetic resonance imaging（ f MRI） scans of T1-weighted images and BOLD brain functional images were performed before and after treatment. Brain regions with differences in local consistency（ Re Ho） and node centrality（ DC） were analyzed. Results Compared with before treatment

Reho values were significantly enhanced in the caudate nucleus

superior temporal anterior cingulate gyrus

anterior cingulate anterior limb

fusiform gyrus

and lateral occipital lobe（ voxel clustering volume > 165 and P < 0. 05）. The DC was significantly enhanced on the back slope

the middle back

the lower back

and the outer side of the pillow（ voxel clustering volume > 165 and P < 0. 05）. Conclusion JIN’s three-needle combined with mirror therapy may promote better reconstruction of brain function in patients with upper limb dysfunction in stroke.