Objective To investigate the effect of Fangfeng（Radix Saposhnikoviae）-Wumei（Fructus Mume）compatibility on airway remodeling in bronchial asthma and its possible mechanism.Methods A total of 20 male Sprague-Dawley rats were randomly divided into a normal group

a Fangfeng group

a Wumei group and a FangfengWumei group（Radix Saposhnikoviae ∶ Fructus Mume = 1 ∶ 1）

with 5 rats in each group.The Fangfeng group

the Wumei group and the Fangfeng-Wumei group were given the corresponding crude drugs of 15.425

15.425

30.850 g/（kg·d） respectively.The normal group was given the same amount of normal saline.Each group was given 3 times a day.Drug-containing serum was prepared 4 days after the intervention.A human bronchial smooth muscle cell（HBSMC） migration model was induced in a manner induced by 20 ng/ml platelet-derived growth factor.The samples divided into a blank control group

a cell model group

a normal serum group

a normal serum cell model group

a hormone intervention group

a Fangfeng serum group

a Wumei serum group

and a Fangfeng-Wumei serum group.HBSMC was inoculated in 5 × 104/ml cell density on plates

with 8 samples in each group.Each group was added with 10% corresponding serum for intervention

37℃

5% CO2 culture.The cell migration rate of each group was observed by cell scratch test at 0

6

and 24 h.After 48 h of culture

the number of transmembrane migration cells was observed by transmembrane migration assay.Matrix metalloproteinase 9（MMP-9） and the concentration of matrix metalloproteinase tissue inhibitor 1（TIMP-1） were detected by ELISA.The MMP-9/TIMP-1 value was calculated.Results Compared with the blank control group and the normal serum group

the cell migration rate increased in the cell model group and the normal serum cell model group at 6 h and 24 h

and the number of transmembrane migration cells also increased at 48 h.The MMP-9 concentration and the MMP-9/TIMP-1 value in the cell supernatant increased and the TIMP-1 concentration decreased（P < 0.05）.Compared with the cell model group and the normal serum cell model group

the above indexes of the Fangfeng-Wumei serum group and the hormone intervention group were significantly improved

and were superior to the Fangfeng serum group and the Wumei serum group（P < 0.05）.Conclusion The Fangfeng（Radix Saposhnikoviae）-Wumei（Fructus Mume） compatibility can significantly inhibit the migration of human bronchial smooth muscle cells and affect airway remodeling

which is better than the use of Radix Saposhnikoviae or Fructus Mume alone.The mechanism may be related to the inhibition of MMP-9/TIMP-1.