Objective To investigate the action characteristics of Xihuang Pills（ 西黄丸） containing rat serum on proliferation of human breast cancer cell. Methods A total of 48 SD rats were randomly divided into a blank control group of 12 rats

a Xihuang Pills 0. 5 h group

Xihuang Pills 1 h group

Xihuang Pills 2 h group and Xihuang Pills 3 h group

with 9 rats in each group. Xihuang Pills groups were given 2. 4 g/（ kg·d） Xihuang Pills suspension

twice a day. The rats in the blank control group were given an equal volume of distilled water for 7 times. Blood samples were collected from the corresponding groups at the last dose of 0. 5

1

2

and 3 hours to prepare drug-containing serum. MDA-MB-435 and MCF-7 cells were respectively set to 10% blank serum group

20% blank serum group

30% blank serum group

10% Xihuang Pills serum group

20% Xihuang Pills serum group and 30% Xihuang Pills serum group

6 duplicate wells per group. After adding the corresponding drug-containing serum

MTT method was used to detect the inhibitory effect of serum containing Xihuang Pills in different blood collection time points and different serum volume fractions on the proliferation of breast cancer cell lines. Results Compared with other time points

for the concentration of drug-containing serum in Xihuang Pills 24 h and 48 h after administration

the drug-containing serum had the strongest inhibitory effect on MDA-MB-435 cells at 2 h after administration. The drug-containing serum had the strongest inhibitory effects on MCF-7 cells at 3 h after 24

48 and 72 h after administration（ P < 0. 05）; and both were 30% Xihuang Pills containing serum had the strongest effects on the proliferation of MDA-MB-435 and MCF-7 cell lines（ P < 0. 05）. Conclusion The 2 h drug-containing serum of Xihuang Pills has the strongest inhibitory effects on MDA-MB-435 cells. The drug-containing serum for 3 h has the strongest inhibitory effect on MCF-7 cells and 30% drug-containing serum had the most strongest inhibitory effects.