Objective To study the potential biological mechanism of Huanglian Jiedu Decoction（ 黄连解毒汤）in the treatment of ischemic stroke by network pharmacology method. Methods Traditional Chinese Medicine Systems Pharmacology（ TCMSP） database was used to obtain effective constituents of Huanglian Jiedu Decoction by screening eligible oral utilization

drug similarity and blood-brain barrier permeability threshold. By drug target prediction and stroke treatment target mining

the 2 target data sets were analyzed to find intersection targets

and the corresponding constituents were used as active constituents. An active constituent target network and an effective constituent target network were constructed by using Cytoscape 3. 6 software. Degree parameters of the effective constituent target network were analyzed to find important effective constituents and targets. Through protein interaction analysis/Kyoto Encyclopedia of Genes and Genomes（ KEGG） enrichment analysis

potential signaling pathways of Huanglian Jiedu Decoction in ischemic stroke were found out. Results A total of 60 active constituents of Huanglian Jiedu Decoction were screened out

including neobaicalein

panicolin

dihydrooroxylin

etc. There were 5 active constituents against cerebral ischemia

including wogonin

β-sitosterol

evodiamine

baicalein and skullcapflavone Ⅰ.And 10 important targets for anti-cerebral ischemia

including androgen receptor

estrogen receptor 1

prostaglandinendoperoxide synthase 2

blood coagulation factor Ⅱ

nitric oxide synthase 3

blood coagulation factor Ⅶ

nuclear receptor 3 C subfamily member 1

5-serotonin receptor 3 A

B lymphocytoma 2 and kinase insert domain-containing receptor（ KDR）. Based on protein interaction network

the important targets of Huanglian Jiedu Decoction were significantly enriched in 7 signaling pathways such as receptor for advanced glycation and products

apoptosis

interleukin17

hypoxia induction factor 1

NOD-like receptors

estrogen and Toll-like receptors. Conclusion Huanglian Jiedu Decoction in ischemic stroke has characteristics like multiple targets

multiple approaches and multiple pathways. Its main biological mechanism may be related to the RAGE-centered signaling pathway.