Objective To investigate the possible mechanism of Yiqi Huatan Quyu Recipe（ 益气化痰祛瘀方） in treating liver injury of obstructive sleep apnea hypopnea syndrome（ OSAHS）. Methods Totally 40 SD rats were randomly divided into normoxic control group

model group

Yiqi Huatan Quyu Recipe low-dose group

and high-dose group

with 10 rats in each group. All groups but normoxic control group were placed in the hypoxic chamber for 8 weeks from 9: 00 to 17: 00 every day to prepare a chronic intermittent hypoxia model. On the first day of modeling

the Yiqi Huatan Quyu Recipe low-dose and high-dose groups were administered with Yiqi Huatan Quyu Recipe 19. 2

38. 4 ml/（ kg·d） respectively

and the normoxic control group and the model group were given 2 ml normal saline by gavage for 8 weeks. HE staining was used to observe the pathological changes of rat liver tissue. Serum aspartate aminotransferase（ AST） and alanine aminotransferase（ ALT） levels were detected. 16 S diversity sequencing was used to detect intestinal microbial composition and relative abundance. Results In the model group

HE staining of the model group showed mild hepatic edema and inflammatory cell infiltration of liver tissue

serum AST and ALT were significantly increased compared with the normoxic control group（ P < 0. 05）. The hepatocytes in the Yiqi Huatan Quyu Recipe high-and low-dose groups were arranged in a strip-like shape

and the inflammatory cells were decreased. The improvement of the Yiqi Huatan Quyu Recipe high-dose group improved more obviously

and AST and ALT were also decreased compared with those of the model group（ P < 0. 05 or P < 0. 01）. 16 S diversity sequencing analysis showed that there were 310 endemic microorganisms in the normoxia control group

112 in the model group

126 in the Yiqi Huatan Quyu Recipe low-dose group

and 138 in the Yiqi Huatan Quyu Recipe high-dose group. The top 5 species in the relative abundance of the rats in each group were Firmicutes

Bacteroides

Proteobacteria

Actinobacteria

TM7

and there was no significant difference in relatively abundance in each group of rats. Compared with the normoxic control group

the model group decreased the Lactobacillus and Braunella at the genus level

and increased in Diphtheria and Corynebacterium（ P < 0. 05）. Lactobacillus and Brouser were increased in the Yiqi Huatan Quyu Recipe low-and high-dose group compared with the model group（ P < 0. 05）. Conclusion Yiqi Huatan Quyu Recipe could affect the microbial species

composition and abundance of intestinal microflora. It may be one of the mechanisms for the treatment of liver injury caused by OSAHS by regulating the production of short-chain fatty acids.