Objective To investigate the effects of Shenqi Compound（参芪复方） on the blood glucose homeostasis of diabetic blood glucose fluctuation model rats and its possible mechanism. Methods A total of 40 spontaneous type 2 diabetic GK rats were randomly divided into a model group

a Shenqi Compound group and a sitagliptin group

with 10 rats in each group. All rats were fed with high-fat diet for 4 weeks to establish a blood glucose fluctuation model. Ten Wistar rats were set into the normal group and fed with ordinary feed for 4 weeks. After successful modeling

the Shenqi Compound group was administered with Shenqi Compound extract 1.44 g/（kg·d）

and the sitagliptin group was administered with 16 mg/（kg·d） of sitagliptin tablet suspension. The normal group

and the model group were given intragastrically with 5 ml/（kg·d） saline for 8 consecutive weeks. Blood was taken from the rat tail vein

and blood glucose levels were measured 5 times during the day at 8:00

10:00

14:00

16:00

and 18:00

and the daily mean blood glucose（MBG） and largest amplitude of glycemic excursions（LAGE） were calculated

standard deviation of mean blood glucose（SDBG） were caculated; real-time fluorescent quantitative PCR was used to detect mRNA expression of biological clock-related genes Id 2

Usp 2

CRY 1

and DBP; 16 S rDNA gene sequencing was used to analyze intestinal flora of each group of rats. Results Compared with the normal group

MBG and SDBG in the other groups were increased

and LAGE in the model group and the Shenqi Compound group was also increased（P<0.01）. Compared with the model group

MBG

SDBG

and LAGE of the Shenqi Compound group and the sitagliptin group were all reduced（P<0.01）. The sitagliptin group was superior to the Shenqi Compound group in reducing SDBG and LAGE（P<0.01）. Compared with the normal group

the expression of Usp 2

CRY1 mRNA and butyric acid levels in the model group rats were reduced（P<0.05 or P<0.01）. Compared with the model group

the rats in the Shenqi Compound group and the sitagliptin group had Id 2 mRNA expression decreased

Usp 2

CRY1 mRNA expression increased

and level of Bacteroides and Butyrica also increased（P<0.05 or P<0.01）; There was no significant difference in the levels of DBP mRNA expression

Broutella

and Rhodium in all groups（P>0.05）. Conclusion Shenqi Compound may regulate the blood glucose homeostasis by regulating the expression of biological clock-related genes and the distribution of intestinal flora in the pancreas tissue of rat models of blood glucose fluctuation.