Objective To evaluate sleep deprivation combined with left anterior descending coronary artery ligation to establish rat model of heart failure with qi deficiency and blood stasis syndrome. Methods A total of 70 SD rats were randomly divided into a blank control group

a model group

a sleep deprivation group

a myocardial infarction group

a Yiqi Huoxue Granule（益气活血颗粒） groups with high

medium and low dosage

with 10 rats in each group. The sleep deprivation group was given irregular sleep deprivation for 8 weeks. The rats in the model group and Yiqi Huoxue Granule group were given 3 weeks of sleep deprivation

and the left anterior descending coronary artery was ligated. The sleep deprivation continued on the 2 nd day after surgery. A model of qi deficiency and blood stasis syndrome of heart failure was established at 5 weeks. Only the left anterior descending coronary artery was ligated in the myocardial infarction group

and the blank control group was fed normally. On the 2 nd day after the operation

Yiqi Huoxue Granules were given gavage at 4.0

2.0

and 1.0 g/（kg·d） in the high

medium

and low-dose groups

and the other groups were given saline 1 ml/100 g

for 5 weeks. The body weight

grasping force

pulse amplitude

autonomic activity

tongue image

cardiac function

ventricular remodeling index

hemorheology index and abdominal aortic blood flow velocity of the rats in each group were compared. Results Compared with the blank control group

the body weight

grasping force

pulse amplitude

and total distance and average speed of autonomous activities of the model group significantly decreased

and the index of ventricular remodeling increased

and the index of cardiac function

wall thickness

and abdominal aortic blood flow velocity significantly decreased. The blood viscosity and plasma viscosity increased significantly under different shear rates

and the relative values of R（red）

G（green）

and B（blue） on the tongue surface were significantly reduced（P<0.05 or P<0.01）. Compared with the model group

the Yiqi Huoxue Granule high-dose group can increase rat weight

grasping force

pulse amplitude

wall thickness

reduce ventricular remodeling

hemorheology indexes

improve rat heart function and autonomous behavior activities

and increase abdominal aorta blood flow velocity

and the relative values of R and G on the tongue surface of rats significantly increased（P<0.05 or P<0.01）. Conclusion The combination of sleep deprivation and left anterior descending coronary artery ligation can induce heart failure rats to show obvious external and objective manifestations of qi deficiency and blood stasis syndrome. The effective intervention of high-dose Yiqi Huoxue Granules contradicts the scientificity and reliability of this model.