Objective To explore the possible mechanism of Xiaopi Granules（消痞颗粒） in treating precancerous lesions of gastric cancer（PLGC）. Method A total of 70 Wistar rats were randomly divided into a blank group of 10 rats

and 60 rats for making models. The modeling group is made by gavage with 200μg/ml of N-methyl-N′-nitro-N-nitrosoguanidine solution 5 ml/（kg·d） each

free drinking of 0.05% ammonia solution and pellet feed containing 0.03% Ranitidine. The 3 factors were combined to establish a rat model of PLGC. At the end of the 44 th week

41 successful modeling rats were randomly divided into a model group（14 rats）

a vitacoenayme group（14 rats）

and a Xiaopi Granules group（13 rats）. Rats in the model group were given normal saline 3 ml/（kg·d） by gavage

the vitacoenayme group was gavage with vitacoenayme tablets 0.3 g/（kg·d）

and the Xiaopi Granules group was gavage with Xiaopi Granules 9 g/（kg·d） for 12 weeks. RT-PCR was used to detect the expression of miR-21 and PTEN gene in gastric mucosa of rats

and phosphoinositide 3-kinase（PI3 K）

protein kinase B（AKT） protein expression in gastric mucosa of rats was detected by ELISA. Results The differences in the expression of miR-21

PTEN gene and AKT

PI3 K protein in gastric mucosal tissue of rats in each group were statistically significant（P<0.01）. Compared with the blank group

the expression of miR-21 gene in the model group and the vitacoenayme group increased

and the expression of PTEN gene decreased（P<0.01）; the expression of AKT and PI3 K protein in the model group

the vitacoenayme group and the Xiaopi Granules group all increased（P<0.01）. Compared with the model group

the expression of miR-21 gene in the Xiaopi Granules group was decreased

the expression of PTEN gene was increased

and the protein expression of AKT and PI3 K were decreased（P<0.01）

but there was no statistical difference in all the indicators between the model group and vitacoenayme group（P>0.05）. Conclusion Xiaopi Granules may regulate the PTEN gene by inhibiting the abnormal expression of miR-21 in gastric mucosal tissue

thereby intervening the PI3 K/AKT signaling pathway to reverse the occurrence of precancerous lesions of gastric cancer.