Objective To explore the protective effect and mechanism of Dangfei Liganning Capsules（当飞利肝宁胶囊） on acute liver injury. Method A total of 30 C57 BL/6 mice were randomly divided into a blank group

a model group and a Dangfei Liganning Capsules group

with 10 mice in each group. Dangfei Liganning Capsules group was given Dangfei Liganning Capsules 0.225 g/（kg·d） by gavage for 10 days

and mice in the blank group and model group were given the same amount of normal saline by gavage. After intervention

mice in the model group and Dangfei Liganning Capsules group were given intraperitoneal injection of D-galactosamine 800 mg/kg to establish acute liver injury models

and mice in the control group were intraperitoneally injected with 100μl of normal saline. Samples were taken 24 hours later to detect liver tissue pathology and liver function including alanine aminotransferase（ALT） and aspartate aminotransferase（AST）

zinc finger protein A20 and tumor necrosis factor α（TNF-α） expression in serum and liver tissue homogenate

expression of zinc finger protein A20

TNF-α

interleukin 1β（IL-1β）

interleukin 6（IL-6）

interleukin 8（IL-8） mRNA and expression of nuclear factor κB p65（NF-κB p65） and phosphorylated nuclear factor κB p65（p-NF-κB p65）

nuclear factor κB inhibitory protein α（IκBα）

phosphorylated nuclear factor κB inhibitory protein α（ p-IκBα） protein in liver tissue. Results Liver histopathological results showed that the infiltration of inflammatory cells in the liver tissue of mice in the model group was significantly increased

while the infiltration of inflammatory cells in the liver tissue of mice in the Dangfei Liganning Capsules group was significantly improved compared to the model group. Compared with the blank group

the serum ALT and AST levels of the model group increased

TNF-α expression in the serum and liver tissue increased

expression of TNF-α

IL-1β

IL-6 mRNA and expression of p-IκBα and p-NF-κB p65 protein in the liver tissue increased（P<0.05 or P<0.01）. Compared with the model group

in Dang Feiliganning group

serum ALT and AST levels decreased

expression of zinc finger protein A20 in the serum and liver tissue increased

TNF-α expression decreased

expression of zinc finger protein A20 mRNA and expression of NF-κB p65 protein increased

TNF-α

IL-1β

IL-6 mRNA

and expression of p-IκBα and p-NF-κB p65 protein in the liver tissue decreased（P<0.05 or P<0.01）. Conclusion Dangfei Liganning Capsules can effectively improve acute liver injury in mice. Up-regulating the expression of zinc finger protein A20 to regulate the activation of NF-κB signaling pathway may be one of its potential mechanisms.