Objective To investigate the effects and mechanism of electroacupuncture on Zusanli（ST36） and Shangjuxu（ST37） on intestinal motility of Yangming fu-viscera excess syndrome. Methods A total of 60 Wistar rats were randomly divided into a control group

a model group and an electro-acupuncture group

with 20 rats in each group. The model group and the electro-acupuncture group were continuously administrated with Chinese drugs of heat nature for 12 days. On the 13 th and 14 th day

the self-fecal suspension was intragastrically administered

and the E. coli bacterial liquid was injected intraperitoneally on the 15 th day to establish a rat model of Yangming Fu-viscera excess syndrome; for the control group

the equal amount of normal saline was intragastrically and intraperitoneally injected. The rats in the electroacupuncture group were electro-acupunctured at bilateral"Zusanli（ST36）" and "Shangjuxu（ST37）" every day

30 minutes each time for 5 days after the successful modeling; the control group and the model group were only bound and fixed without intervention. After the intervention was completed

the frequency and amplitude of myoelectric slow wave in the upper small intestine of rats are detected to assess the changes in small intestine motor function; the upper small intestine tissue samples were taken for HE staining to observe the pathological damage of the small intestine; the enteric glial cells were observed by immunofluorescence confocal microscope and transmission electron microscope changes in morphology and ultrastructure. Results Compared with the control group

the small intestine slow wave frequency and amplitude of rats in the model group were slowed down（P<0.05 or P<0.01）; small intestine pathological damage was obvious

enteric glial cell morphology was abnormal

network structure disorder

fluorescence intensity OD value decreased（P<0.05）

transmission electron microscope observation showed enteric glial cell chromatin aggregation

uneven distribution

mitochondria swelling; in the electroacupuncture group

the small intestinal slow wave frequency increased

the amplitude increased（P<0.05）

and the small intestine pathological damage and enteric glial cells and network structure damage were reduced

the fluorescence intensity OD value increased（P<0.05）

and enteric glial cell ultrastructure damage was improved. Conclusion Electro-acupuncture "Shangjuxu（ST36）" and "Zusanli（ST37）" can promote the recovery of small intestine motor function in Yangming Fu-viscera excess syndrome rats

and the mechanism may be related to repairing the damage of enteric glial cell network.