Objective To explore the anti-tumor medicine in Shennong′s Classic of the Materia Medica（《神农本草经》） by analyzing their natures

flavors

channel entry

and modern pharmacological components

and to provide references for researches and development of anti-tumor medicine. Methods Keywords related to concretions and conglomerations（癥瘕） and accumulations and gatherings（积聚） were used to identify tumor-related medicine in Shennong′s Classic of the Materia Medica. The identified medicine were then standardized and deleted according to Chinese Pharmacopoeia（《中华人民共和国药典》）. Chinese Material Medica（《中药学》） was referred to when classifying the standardized medicine

and the four qi

five flavors

and channel entry of the medicine were calculated. TCMSP platform and TCMID platform were used to search the compounds of the medicine

and Cytoscape 3.7.2 software was adopted to analyze the medicine and its compounds. Results Seventy-three medicine were found to be related with concretions

conglomerations

accumulations and gatherings in Shennong′s Classic of the Materia Medica

of which lower grade and botanicals were the most

followed by middle grade and animal medicine. After standard deletion

42 commonly used anti-tumor medicine were included

mostly of bitter-cold flavor followed by acrid-warm flavor

and entering lung

spleen

liver

kidney and stomach channel. The analysis by Cytoscape 3.7.2 software showed that the top 10 medicine of highest degree value were Mahuang（Herba Ephedrae）

Chishao（Radix Paeoniae Rubra）

Gandihuang（Radix Rehmanniae Recens）

Sangbaipi（Cortex Mori）

Mudanpi（Cortex Moutan）

Xiakucao（Spica Prunellae）

Jingdaji（Radix Euphorbiae Pekinensis）

Chaihu（Radix Bupleuri）

Danshen（Radix et Rhizoma Salviae Miltiorrhizae）

and Baitouweng（Radix Pulsatillae）; the top five compounds were β-sitosterol

quercetin

kaempferol

sitosterol

and luteolin. Conclusion The properties

natures

flavors

and channel entries of anti-tumor medicine in Shennong′s Classic of the Materia Medica are in line with the basic idea of tumor treatment; the high frequency Chinese medicine and compounds have been confirmed to have certain anti-tumor effects in modern studies

providing theoretical references for the development and research of anti-tumor medicine.