Objective

2

To evaluate the effect of

Jiaotai Pills

（交泰丸） on the sleep stage and sleep quality in insomnia model rats with syndrome of non-interaction between the heart and the kidney.

Methods

2

Twenty-one SD rats were randomly divided into control group， model group and

Jiaotai Pills

group， with 7 rats in each group. Except for the control group， the rat model of insomnia with non-interaction between the heart and the kidney was established through compound stimulation and intraperitoneal injection of para-chlorophenylalanine （PCPA）. After modeling， the

Jiaotai Pills

group was administrated with 1.1 g/kg of

Jiaotai Pills

by gavage， while the control group and model group were given 10 mL/kg of distilled water by gavage， once a day for 7 consecutive days. Electroencephalogram（EEG） and electromyogram （EMG） were recorded through animal sleep bioanalytical system. The total amount of night ［（19：00-07：00 （+1）］ and day ［（07：00（+1）-19：00（+1）］ wake， non-rapid eye movement （NREM） sleep and rapid eye movement （REM） sleep， the number of sleep stage transitions， the episode number of NREM sleep， REM sleep and wake， and EEG power density were analyzed. After recording， the adrenal index was determined， and the adrenocorticotropic hormone （ACTH） and corticotropin-releasing hormone （CRH） levels in serum were detected through ELSA.

Results

2

Compared to those in the control group， the adrenal index and serum ACTH level in the model group significantly increased （

P

＜0.05 or

P

＜0.01）， while there was no difference between groups in CRH level （

P

＞0.05）； the total amount of night and day NREM sleep and REM sleep were reduced， while the wake amount increased （

P

＜0.05 or

P

＜0.01）， with increased episode number of 10-20 s and 30-50 s NREM sleep and decreased 120-230 s and 240-470 s NREM sleep （

P

＜0.05 or

P

＜0.01）， as well as increased number of NREM sleep transiting into wake and decreased number of NREM sleep transiting into REM sleep and that of REM sleep transiting into wake （

P

＜0.05 or

P

＜0.01）. Compared to those in the model group， the adrenal index and ACTH level in serum in the

Jiaotai Pills

group were reduced （

P

＜0.05）， but there was no difference in CRH （

P

＞0.05）； the total amount of night and day NREM sleep increased while the wake amount was reduced significantly （

P

＜0.01）， with decreased episode number of 10-20 s and 30-50 s NREM sleep and increased episode number of 120-230 s and 240-470 s NREM sleep （

P

＜0.01）， as well as reduced number of NREM sleep transiting into wake （

P

＜0.01）. There were no significant differences in the Delta wave density of NREM sleep and the Theta wave density of REM sleep between the

Jiaotai Pills

group and control group （

P

＞0.05）.

Conclusion

2

Jiaotai Pills

can improve sleep stage and sleep quality mainly by increasing long duration NREM sleep （120-230 s and 240-470 s） and reducing the transition number between NREM sleep and wake， and the induced sleep is similar to physiological sleep.