Objective

2

To explore the modeling method of diarrhea mice with kidney-

yang

depletion syndrome.

Methods

2

Model building stage： Twenty male Kunming mice were randomly divided into the blank group and modeling group， with 10 mice in each group. Mice in the modeling group were given daily gavage of 50 mg/（kg·d）adenine suspension for 14 days. From the 8th day onwards， the modeling group was gavaged with 10 g/（kg·d）of Fanxieye （

Folium sennae）

decoction， once a day for 7 days. Mice in the blank group were given 0.4 ml sterile water daily by gavage for 14 days. The general behavior of the mice in both groups was observed， while the body weight and rectal temperature were measured， and the diarrhea index was calculated on the 1st， 5th， 9th， and 13th day of modeling. At the end of the 14th day of modeling， the serum content of cyclic adenosine monophosphate （cAMP）and cyclic guanosine monophosphate （cGMP）were tested， and the cAMP/cGMP value was calculated. Model validation stage： the remaining 30 mice were randomly assigned to the normal group， model control group and

Sishen Pill

（四神丸） group， with 10 mice in each group. The model was built in the model control group and

Sishen Pill

group with the above mentioned method. After successful modeling， mice in the

Sishen Pill

group were gavaged with 5 g/（kg·d）of

Sishen Pill

decoction， twice a day for 7 days. Mice in the normal and model control groups were given 0.35 ml sterile water by gavage， twice a day for 7 days. Mice were tested for general behaviour， and body weight， rectal temperature， and diarrhea index were assessed on the 1st， 3th， and 7th day. The serum content of cAMP and cGMP and the cAMP/cGMP value were assessed after 7 days.

Results

2

During the model building stage， the mental state and autonomous activity of the mice in the blank group were normal， and the perianal region was clean with soft， formed and moderate watery stool. Mice in the modeling group were in poor mental state， with cool body and cold tail， light nails， sparse and dull hair， and soft feces. Compared to those in the normal group， the body weight of the mice in the modeling group decreased on the 9th and 13th days of modeling， and the rectal temperature of the mice decreased on the 5th， 9th， and 13th days （

P

＜0.01）. As the modeling time increased， the diarrhea index in the modeling group increased in a time-dependent manner； in the modeling group， the serum cAMP content decreased， and the cGMP content increased， with decreased cAMP/cGMP value （

P

＜0.01）. During the model validation stage， the mental state of the mice in the

Sishen Pill

group were improved compared to the model control group， and the movement of the mice was flexible， with improved gloss of the fur， the color of the nails and the loose feces. Compared to the model control group， the diarrhea index of the

Sishen Pill

group on the 3rd and 7th days of treatment significantly decreased （

P

＜0.05）. There was no significant difference in serum cAMP， cGMP and cAMP/cGMP values between groups after 7 days （

P

＞0.05）.

Conclusion

2

Adenine combined with Fanxieye （

Folium sennae）

decoction can be successfully used to establish the animal model of diarrhea with kidney-

yang

depletion syndrome， in accordance with its clinical characteristics.