Objective

2

To explore the possible mechanism of

Danggui Sini Granules

（当归四逆颗粒） in treatment of coronary heart disease with cold congealing and blood stasis syndrome.

Methods

2

Fifty SD rats were randomly divided into a blank group， a model group， a

Danshen Pill

（丹参滴丸） group， and a low- and high-dosage

Danggui Sini Granules

group， with 10 rats in each group. Except for the blank group， the rat model of coronary heart disease with cold congealing and blood stasis syndrome was established by repeated cold stimulation at low temperature combined with intraperitoneal injection of posterior pituitary hormone in all other groups. In the 6th week of modelling， 0.073 g·kg

-1

·d

-1

of compound

Danshen Pill

was given to the

Danshen Pill

group， 20.2 and 40.4 g·kg

-1

·d

-1

of

Danggui Sini Granules

were given to the low- and high-dose

Danggui Sini Granules

groups， respectively， and 0.2 ml/10 g of sterile water was given to the blank group and the model group， all for 2 consecutive weeks. The general conditions of the rats were recorded， and the body mass was compared weekly. At the end of the intervention， electrocardiogram， blood rheological indexes， including whole blood low-cut viscosity， whole blood high-cut viscosity， erythrocyte aggregation index， and cardiac index were detected to evaluate the effect of the medication， and HE staining was used to observe the myocardial histopathological changes， TUNEL staining to detect the apoptotic situation of cardiomyocytes， and ELISA to detect the serum thyroid-stimulating hormone （TSH）， free triiodothyronine （FT

3

）， free tetraiodothyronine （FT

4

） levels and thromboxane B

2

（TXB

2

）， 6-keto-prostaglandin F1α （6-Keto-PGF

1α

）， endothelin 1 （ET-1）， and n

itric oxide （NO） levels.

Results

2

Compared with the blank group at the same time point， the body mass of rats in the remaining groups decreased at all time points （

P

＜0.05 or

P

＜0.01）； compared with the model group at the same time point， the body mass of rats in high-dose

Danggui Sini Granules

group and

Danshen Pill

group increased at the 6th and 7th weeks （

P

＜0.05 or

P

＜0.01）. In the blank group， the S-T segment of the electrocardiogram was close to the isoelectric line， the myocardial structure was regular， the fibres were closely arranged， and the nuclei of the cells were intact and neatly aligned； in the model group， the S-T segment of the electrocardiogram significantly elevated， and the arrangement of the myocardial fibres was obviously disordered， with myocardial cells appearing to be swollen， necrotic， and infiltrated by inflammatory cells， and the apoptosis-positive cells of the cardiac muscle cells obviously increased； in each of the medication groups， the electrocardiogram had a lowered S-T segment， and myocardial fibres were aligned， myocyte structure and morphology were improved， inflammatory cells reduced， and the number of apoptosis-positive cells significantly reduced. Compared with the blank group， the cardiac index， whole blood high cut viscosity， whole blood low cut viscosity， erythrocyte aggregation index increased in the model group， and the serum levels of TSH， FT

3

， FT

4

， NO， 6-Keto-PGF

1α

significantly reduced， and the levels of ET-1 and TXB

2

significantly increased （

P

＜0.05 or

P

＜0.01）. Compared with the model group， the levels of cardiac index， whole blood high cut viscosity， whole blood low cut viscosity， erythrocyte aggregation index， ET-1 and TXB

2

significantly decreased and the levels of NO and 6-Keto-PGF

1α

significantly increased in each medication group， and the

serum levels of TSH， FT

3

， and FT

4

elevated in the high-dose

Danggui Sini Granules

group and

Danshen Pill

group （

P

＜0.05 or

P

＜0.01）. The serum TSH level of rats in the low-dose

Danggui Sini Granules

group was lower than that in

Danshen Pill

group and the high-dose

Danggui Sini Granules

group （

P

＜0.01）.

Conclusion

2

Danggui Sini Granules

have the effect of alleviating myocardial injury in coronary heart disease with cold congealing and blood stasis syndrome， and its mechanism may be related to reducing myocardial cell apoptosis， improving energy metabolism， and regulating the level of vasoactive factors.