1.天津中医药大学实验针灸学研究中心,天津市静海区团泊新城西区鄱阳湖路10号,301617
2.青海大学
18394675118@163.com
收稿:2025-09-23,
修回:2026-04-28,
录用:2026-04-02,
纸质出版:2026-05-17
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李峻毅,涂世伟,刘阳阳等.针刺穴位效应启动的物理生物学研究概况[J].中医杂志,2026,67(10):1130-1136.
LI Junyi,TU Shiwei,LIU Yangyang,et al.Overview of Physical Biological Research on the Activation of Acupoint Effects by Acupuncture[J].Journal of Traditional Chinese Medicine,2026,67(10):1130-1136.
李峻毅,涂世伟,刘阳阳等.针刺穴位效应启动的物理生物学研究概况[J].中医杂志,2026,67(10):1130-1136. DOI: 10.13288/j.11-2166/r.2026.10.018.
LI Junyi,TU Shiwei,LIU Yangyang,et al.Overview of Physical Biological Research on the Activation of Acupoint Effects by Acupuncture[J].Journal of Traditional Chinese Medicine,2026,67(10):1130-1136. DOI: 10.13288/j.11-2166/r.2026.10.018.
通过梳理针刺穴位效应启动的物理生物学研究,从针刺机械力学信息的产生、转导角度阐释针刺穴位效应启动机制,揭示了穴区微环境中物理-化学耦合过程。提出未来研究应聚焦于局部机械力敏感性细胞,深入探究针刺力学信号如何引发穴区细胞与分子的动态变化及其物理-化学信息转导机制,为针刺穴位效应启动提供科学依据。相关研究将有助于深化针刺作用原理的科学内涵,推动针灸的临床应用与发展。
By reviewing the physical biological research on the activation of acupoint effects by acupuncture, this paper explains the activation mechanism from the perspective of the generation and transmission of mechanical signals caused by acupuncture, and reveals the physical-chemical coupling processes in the acupoint microenvironment. Future research should focus on locally mechanosensitive cells, further exploring how acupuncture mechanical signals trigger dynamic changes in cells and molecules in the acupoints, and the physical-chemical information transduction mechanism, which will provide scientific evidence for the acupoint activation during acupuncture. Related studies will contribute to a deeper understanding of the scientific principles behind acupuncture and promote its clinical application and development.
WEICHSELBERGER V , MOORE G , TLILI S , et al . Biophysics of organoids [J]. Developmental Cell , 2026 , 61 ( 1 ): 24 - 41 .
NELSON C M , XIAO B , WICKSTR M S A , et al . Mechanobiology: shaping the future of cellular form and function [J]. Cell , 2024 , 187 ( 11 ): 2652 - 2656 .
GARCIA H G , BREWSTER R C , PHILLIPS R . Using synthetic biology to make cells tomorrow's test tubes [J]. Integr Biol(Camb) , 2016 , 8 ( 4 ): 431 - 450 .
杨馥铭 , 徐枝芳 , 周丹 . 物理生物学:大科学观下针灸学的突破 [J]. 中华中医药杂志 , 2019 , 34 ( 3 ): 896 - 900 .
LI N C , LI M Y , CHEN B , et al . A new perspective of acupuncture: the interaction among three networks leads to neutralization [J]. Evid Based Complement Alternat Med , 2019 , 2019 : 2326867 . doi: 10.1155/2019/2326867 http://dx.doi.org/10.1155/2019/2326867 .
GONG Y , LI N , LV Z , et al . The neuro-immune microenvironment of acupoints-initiation of acupuncture effectiveness [J]. J Leukoc Biol , 2020 , 108 ( 1 ): 189 - 198 .
LI N , GUO Y , GONG Y , et al . The anti-inflammatory actions and mechanisms of acupuncture from acupoint to target organs via neuro-immune regulation [J]. J Inflamm Res , 2021 , 14 : 7191 - 7224 . doi: 10.2147/JIR.S341581 http://dx.doi.org/10.2147/JIR.S341581 .
薛玺情 , 曾以德 . 从穴区细胞对针刺时的响应浅谈手针针刺启动 [J]. 中华中医药杂志 , 2022 , 37 ( 9 ): 5248 - 5252 .
LANGEVIN H M , YANDOW J A . Relationship of acupuncture points and meridians to connective tissue planes [J]. Anat Rec , 2002 , 269 ( 6 ): 257 - 265 .
LANGEVIN H M , KONOFAGOU E E , BADGER G J , et al . Tissue displacements during acupuncture using ultrasound elastography techniques [J]. Ultrasound Med Biol , 2004 , 30 ( 9 ): 1173 - 1183 .
KIMURA M , TOHYA K , KUROIWA K , et al . Electron microscopical and immunohistochemical studies on the induction of "Qi" employing needling manipulation [J]. Am J Chin Med , 1992 , 20 ( 1 ): 25 - 35 .
李程 , 陈波 , 胡铁汉 , 等 . 不同规格毫针针刺对大鼠穴区筋膜组织、细胞和胶原纤维的影响 [J]. 中国针灸 , 2015 , 35 ( 8 ): 801 - 805 .
LANGEVIN H M , CHURCHILL D L , WU J , et al . Evidence of connective tissue involvement in acupuncture [J]. FASEB J , 2002 , 16 ( 8 ): 872 - 874 .
李孝光 , 房台生 , 党汝霖 . 穴位与运动点是否存在对应关系 [J]. 西安交通大学学报(医学版) , 1981 ( 2 ): 204 - 209 .
TIAN D S , XIONG J , PAN Q , et al . De qi, a threshold of the stimulus intensity, elicits the specific response of acupoints and intrinsic change of human brain to acupuncture [J]. Evid Based Complement Alternat Med , 2014 , 2014 : 914878 . doi: 10.1155/2014/914878 http://dx.doi.org/10.1155/2014/914878 .
LANGEVIN H M , CHURCHILL D L , CIPOLLA M J . Mechanical signaling through connective tissue: a mechanism for the therapeutic effect of acupuncture [J]. FASEB J , 2001 , 15 ( 12 ): 2275 - 2282 .
LANGEVIN H M , BOUFFARD N A , BADGER G J , et al . Dynamic fibroblast cytoskeletal response to subcutaneous tissue stretch ex vivo and in vivo [J]. Am J Physiol Cell Physiol , 2005 , 288 ( 3 ): C747 - C756 .
陈波 , 王兴桂 , 李小玉 , 等 . 体外压力刺激对大鼠筋膜组织成纤维细胞形态和MMP-1、MMP-3合成释放影响的研究 [J]. 辽宁中医杂志 , 2011 , 38 ( 4 ): 770 - 772 .
XU S B , HUANG B , ZHANG C Y , et al . Effectiveness of strengthened stimulation during acupuncture for the treatment of Bell palsy: a randomized controlled trial [J]. CMAJ , 2013 , 185 ( 6 ): 473 - 479 .
李姝 , 宋思敏 , 李玲 , 等 . 得气的量化评价及客观表征研究现状概述 [J]. 上海针灸杂志 , 2023 , 42 ( 12 ): 1331 - 1336 .
张迪 , 丁光宏 , 沈雪勇 , 等 . 针刺引起结缔组织效应的研究进展 [J]. 针刺研究 , 2004 , 29 ( 1 ): 77 - 81 .
WANG F , CUI G W , KUAI L , et al . Role of acupoint area collagen fibers in anti-inflammation of acupuncture lifting and thrusting manipulation [J]. Evid Based Complement Alternat Med , 2017 , 2017 : 2813437 . doi: 10.1155/2017/2813437 http://dx.doi.org/10.1155/2017/2813437 .
余晓佳 , 丁光宏 , 姚伟 , 等 . 穴位处胶原纤维在针刺大鼠“足三里”镇痛过程中的作用 [J]. 中国针灸 , 2008 , 28 ( 3 ): 207 - 213 .
何伟 , 吴美玲 , 景向红 , 等 . 穴位的本态:穴位组织细胞化学的动态变化 [J]. 中国针灸 , 2015 , 35 ( 11 ): 1181 - 1186 .
CHEN Y , GUO Y , WANG J , et al . Fractal characterization of acupuncture-induced spike trains of rat WDR neurons [J]. Chaos Soliton Fract , 2015 , 77 : 205 - 214 . doi: 10.1016/j.chaos.2015.05.026 http://dx.doi.org/10.1016/j.chaos.2015.05.026 .
熊江艳 , 陈波 , 刘峰 , 等 . 张力刺激对经脉筋膜成纤维细胞整合素β1亚基影响的体外研究 [J]. 江西中医药 , 2015 , 46 ( 10 ): 31 - 33 .
潘萍 , 郭义 . 肥大细胞是针刺效应信号放大的关键因素之一 [J]. 辽宁中医杂志 , 2009 , 36 ( 12 ): 2066 - 2068 .
康婧青 , 张赛 , 郭义 , 等 . 穴位局部P物质与肥大细胞在针刺效应启动中作用的研究 [J]. 上海针灸杂志 , 2014 , 33 ( 11 ): 1054 - 1058 .
COSGROVE B D , BOUNDS L R , TAYLOR C K , et al . Mechanosensitive genomic enhancers potentiate the cellular response to matrix stiffness [J]. Science , 2025 , 390 ( 6778 ): eadl1988 .
MAKKAR H , TRAN N , CHEN Y C , et al . Matrix stiffness governs fibroblasts' regulation of gingival immune homeostasis [J]. Adv Mater , 2026 , 38 ( 15 ): e20717 .
张志英 , 党瑞山 , 张传森 , 等 . 大鼠后三里针刺前后细胞外基质的差异性基因表达 [J]. 解剖学杂志 , 2007 , 30 ( 2 ): 143 - 145 .
王学新 , 李永春 , 马晓芃 , 等 . 针刺颈夹脊穴对大鼠退变颈椎间盘细胞外基质金属蛋白酶1、3和金属蛋白酶抑制因子1的影响 [J]. 针刺研究 , 2009 , 34 ( 4 ): 248 - 251 .
孙卫强 , 张光志 . 针刺对颈椎间盘退变大鼠椎间盘VEGF、PLA2和PGE2和细胞外基质胶原、代谢酶表达的影响 [J]. 海南医学院学报 , 2019 , 25 ( 17 ): 1308 - 1313 .
CONWAY J R W , ISOMURSU A , FOLLAIN G , et al . Defined extracellular matrix compositions support stiffness-insensitive cell spreading and adhesion signaling [J]. Proc Natl Acad Sci U S A , 2023 , 120 ( 43 ): e2304288120 .
KERSEY A L , CHENG D Y , DEO K A , et al . Stiffness assisted cell-matrix remodeling trigger 3D mechanotransduction regulatory programs [J]. Biomaterials , 2024 , 306 : 122473 . doi: 10.1016/j.biomaterials.2024.122473 http://dx.doi.org/10.1016/j.biomaterials.2024.122473 .
JIANG C , CENTONZE A , SONG Y , et al . Collagen signaling and matrix stiffness regulate multipotency in glandular epithelial stem cells in mice [J]. Nat Commun , 2024 , 15 ( 1 ): 10482 .
ADEBOWALE K , ALLAN C , HA B , et al . Monocytes use protrusive forces to generate migration paths in viscoelastic collagen-based extracellular matrices [J]. Proc Natl Acad Sci U S A , 2025 , 122 ( 25 ): e2309772122 .
CHAUDHURI O , COOPER-WHITE J , JANMEY P A , et al . Effects of extracellular matrix viscoelasticity on cellular behaviour [J]. Nature , 2020 , 584 ( 7822 ): 535 - 546 .
TU S W , KAWANOKUCHI J , TAKAGI K , et al . Fibroblasts as key effectors of acupuncture in treatment of rheumatoid arthritis [J]. Front Immunol , 2026 , 17 : 1715313 . doi: 10.3389/fimmu.2026.1715313 http://dx.doi.org/10.3389/fimmu.2026.1715313 .
KREGER S T , VOYTIK-HARBIN S L . Hyaluronan concentration within a 3D collagen matrix modulates matrix viscoelasticity, but not fibroblast response [J]. Matrix Biol , 2009 , 28 ( 6 ): 336 - 346 .
VAN STEEN A C I , GRONLOH M L B , JOOSTEN S , et al . Endothelial ICAM-1 adhesome recruits CD44 for optimal transcellular migration of human CTLs [J]. J Immunol , 2023 , 211 ( 3 ): 377 - 388 .
ALBTOUSH N , QUEISSER K A , ZAWERTON A , et al . TSG6 hyaluronan matrix remodeling dampens the inflammatory response during colitis [J]. Matrix Biol , 2023 , 121 : 149 - 166 . doi: 10.1016/j.matbio.2023.06.007 http://dx.doi.org/10.1016/j.matbio.2023.06.007 .
LIU L , DENG D , LI C , et al . The combination of modified acupuncture needle and melittin hydrogel as a novel therapeutic approach for rheumatoid arthritis treatment [J]. J Nanobiotechnology , 2024 , 22 ( 1 ): 432 .
SARASWATHIBHATLA A , INDANA D , CHAUDHURI O . Cell-extracellular matrix mechanotransduction in 3D [J]. Nat Rev Mol Cell Biol , 2023 , 24 ( 7 ): 495 - 516 .
张欢 , 赵国清 , 冯锦腾 , 等 . 力敏感受体介导细胞功能调控的力学生物学研究 [J]. 力学进展 , 2023 , 53 ( 1 ): 48 - 153 .
齐颖新 . 细胞核与应力信号转导 [J]. 医用生物力学 , 2022 , 37 ( 3 ): 385 - 388 .
韦堂墙 , 牛超 , 伍季 , 等 . 细胞对机械应力的感知及其信号转导机制 [J]. 华西医学 , 2022 , 37 ( 3 ): 449 - 452 .
BISWAS K H . Molecular mobility-mediated regulation of E-cadherin adhesion [J]. Trends Biochem Sci , 2020 , 45 ( 2 ): 163 - 173 .
BOCK F , LI S , POZZI A , et al . Integrins in the kidney- beyond the matrix [J]. Nat Rev Nephrol , 2025 , 21 ( 3 ): 157 - 174 .
CONWAY J R W , JOSHI O , KAIVOLA J , et al . Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells [J]. Nat Cell Biol , 2025 , 27 ( 6 ): 1021 - 1034 .
BARCELONA-ESTAJE E , OLIVA M A G , CUNNIFFE F , et al . N-cadherin crosstalk with integrin weakens the molecular clutch in response to surface viscosity [J]. Nat Commun , 2024 , 15 ( 1 ): 8824 .
KANCHANAWONG P , CALDERWOOD D A . Organi-zation, dynamics and mechanoregulation of integrin-mediated cell-ECM adhesions [J]. Nat Rev Mol Cell Biol , 2023 , 24 ( 2 ): 142 - 161 .
陈波 . 模拟毫针机械刺激诱导成纤维细胞压力信号生物转化作用与针刺效应的研究 [D]. 成都 : 成都中医药大学 , 2005 .
刘峰 . 体外不同力学刺激对经脉相关筋膜成纤维细胞整合素-细胞骨架信号传导通路影响的研究 [D]. 贵阳 : 贵阳中医学院 , 2013 .
刘峰 , 胡铁汉 , 罗明鸿 , 等 . 体外不同压力刺激对筋膜成纤维细胞整合素β1亚基和微丝表达的影响 [J]. 贵阳中医学院学报 , 2016 , 38 ( 1 ): 6 - 10 .
GUO Y , LI Y , XU T , et al . An inspiration to the studies on mechanisms of acupuncture and moxibustion action derived from 2021 Nobel Prize in Physiology or Medicine [J]. Acupunct Herb Med , 2022 , 2 ( 1 ): 1 - 8 .
ZHANG M , MA Y , YE X , et al . TRP (transient receptor potential) ion channel family: structures, biological functions and therapeutic interventions for diseases [J]. Signal Transduct Target Ther , 2023 , 8 ( 1 ): 261 .
XIAO B . Mechanisms of mechanotransduction and phy-siological roles of PIEZO channels [J]. Nat Rev Mol Cell Biol , 2024 , 25 ( 11 ): 886 - 903 .
RASH L D , KELLENBERGER S . Acid-sensing ion channels: structure, function, pharmacology, and clinical significance [J]. Physiol Rev , 2026 , 106 ( 1 ): 281 - 362 .
LIU Y , JIANG B J , ZHAO R Z , et al . Epithelial sodium channels in pulmonary epithelial progenitor and stem cells [J]. Int J Biol Sci , 2016 , 12 ( 9 ): 1150 - 1154 .
ANDRINI O , SOUSSIA IBEN , TARDY P , et al . Constitutive sodium permeability in a C. elegans two-pore domain potassium channel [J]. Proc Natl Acad Sci U S A , 2024 , 121 ( 43 ): e2400650121 .
HUANG M , WANG X , XING B , et al . Critical roles of TRPV2 channels, histamine H1 and adenosine A1 receptors in the initiation of acupoint signals for acupuncture analgesia [J]. Sci Rep , 2018 , 8 ( 1 ): 6523 .
CHEN Z , YAO K , WANG X , et al . Acupuncture promotes muscle cells ATP metabolism in ST36 acupoint local exerting effect by activating TRPV1/CaMKⅡ/AMPK/PGC1α signaling pathway [J]. Chin Med , 2025 , 20 ( 1 ): 112 .
YAO K , CHEN Z , LI Y , et al . TRPA1 ion channel mediates the analgesic effects of acupuncture at the ST36 acupoint in mice suffering from arthritis [J]. J Inflamm Res , 2024 , 17 : 1823 - 1837 . doi: 10.2147/JIR.S455699 http://dx.doi.org/10.2147/JIR.S455699 .
郑雅文 . 穴区机械敏感性TRPA1与TRPV4蛋白在针刺镇痛外周机制中的作用 [D]. 上海 : 上海中医药大学 , 2020 .
王江山 . 基于单细胞测序的穴区成纤维细胞机械敏感离子通道Piezo1介导针刺镇痛效应机制研究 [D]. 天津 : 天津中医药大学 , 2025 .
翟永晓 . 基于Piezo2激活牵张反射介导针刺得气客观反应的机制研究 [D]. 天津 : 天津中医药大学 , 2024 .
朱海宁 , 张迪 , 黄猛 , 等 . 肥大细胞对针灸治疗过程中的物理刺激的敏感性机制研究 [J]. 复旦学报(自然科学版) , 2013 , 52 ( 5 ): 681 - 687 .
李亚男 , 徐枝芳 , 吕中茜 , 等 . 损伤相关分子:针刺起效过程中的“前哨兵” [J]. 针灸临床杂志 , 2022 , 38 ( 5 ): 1 - 5 .
ZINDEL J , KUBES P . DAMPs, PAMPs, and LAMPs in immunity and sterile inflammation [J]. Annu Rev Pathol , 2020 , 15 : 493 - 518 . doi: 10.1146/annurev-pathmechdis-012419-032847 http://dx.doi.org/10.1146/annurev-pathmechdis-012419-032847 .
MA M , JIANG W , ZHOU R . DAMPs and DAMP-sensing receptors in inflammation and diseases [J]. Immunity , 2024 , 57 ( 4 ): 752 - 771 .
XU Y , WANG Y , PARK G , et al . Non-specific physiological background effects of acupuncture revealed by proteomic analysis in normal rats [J]. BMC Complement Altern Med , 2014 , 14 : 375 . doi: 10.1186/1472-6882-14-375 http://dx.doi.org/10.1186/1472-6882-14-375 .
GOLDMAN N , CHEN M , FUJITA T , et al . Adenosine A1 receptors mediate local anti-nociceptive effects of acupuncture [J]. Nat Neurosci , 2010 , 13 ( 7 ): 883 - 888 .
窦报敏 . 脊髓CXCL1-CXCR2介导针刺足三里抗炎性痛的作用及机制研究 [D]. 天津 : 天津中医药大学 , 2020 .
李艳伟 , 窦报敏 , 王慎军 , 等 . S1脑区CXCL1/CXCR2参与针刺改善佐剂性关节炎大鼠炎性痛的作用机制 [J]. 针刺研究 , 2024 , 49 ( 4 ): 331 - 340 .
康晓春 . 针刺足三里诱导的C1q对穴区HMGB1-RAGE-NFκB信号轴的调控作用及机制研究 [D]. 天津 : 天津中医药大学 , 2025 .
YANG J , WANG Z , JIANG J , et al . Effects of acupuncture on serotonin, histamine, substance P, and tryptase levels at sensitized points in model rats with knee osteoarthritis [J]. J Tradit Chin Med Sci , 2024 , 11 ( 4 ): 500 - 512 .
杜思敏 , 林小伟 , 陈志翰 , 等 . 成纤维细胞与巨噬细胞的胞间通讯在针刺穴区启动机制中的作用探讨 [J]. 上海针灸杂志 , 2025 , 44 ( 5 ): 631 - 638 .
薛玺情 , 曾以德 . 基于肥大细胞与成纤维细胞的相互作用探讨手针针刺启动机制 [J]. 中华中医药杂志 , 2024 , 39 ( 4 ): 1666 - 1670 .
魏斐 . 剪切力作用下的肥大细胞内钙信号和脱颗粒研究 [D]. 上海 : 复旦大学 , 2011 .
TU S , LI Y , LI J , et al . Mechanical stretch-mediated fibroblast activation: the pivotal role of Piezo1 channels [J]. Biochim Biophys Acta Mol Cell Res , 2025 , 1872 ( 7 ): 120008 .
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