Objective:To explore the relation of the spleen and stomach damp-heat syndrome with AQP（aquaporin）3 and AQP4 expressions

and the mechanism of the action of Qingre Huashi Prescription.Methods:68 cases of chronic superficial gastritis

including 53 cases of spleen and stomach damp-heat syndrome（19 cases with more heavy dampness

14 cases with more heavy heat

20 cases with equal dampness and heat）

15 cases with spleen deficiency syndrome

10 cases with normal findings by gastroscopy used as normal group.The patients with spleen and stomach damp-heat syndrome were treated with Qingre Huashi Prescription

and AQP3 and AQP4 expressions were detected with immunohistochemical method.Results:AQP3 and AQP4 expressions in the spleen and stomach damp-heat group were significantly stronger than those in the normal group（P<0.05）.There was no significant difference between the spleen deficiency group and the normal group（P>0.05）;and there was no significant difference among the subgroups（P>0.05）.After treatment

the expressions of AQP3 and AQP4 in the spleen and stomach damp-heat syndrome group decreased significantly（P<0.01）

with no significant difference as compared with the normal group and the spleen deficiency group.Conclusion:Abnormal expression of AQP3 and AQP4 is possibly one of the mechanisms of spleen and stomach damp-heat syndrome generation

and Qingre Huashi Prescription exerts therapeutic effect possibly through influencing expression of AQP3 and AQP4.