Objective To explore the effect and mechanism of Bushen Qiangji Granules（Granules for reinforcing the kidney to strengthen the spine） on ankylosing spondylitis（AS）.Methods The fibroblasts of AS hip-joint capsule cultured in vitro

as the experimental subjects

were divided into normal group

AS control group

AS cell factor induction group（AS induction group）

AS rabbit blank serum group

Bushen Qiangji Granules small-dose

medium-dose

and large-dose groups

and salicylazosulfapyridine（SASP） control group.Through the induction of bone morphogenetic protein（BMP） and transformation growth factor-β（TGF-β）

the effect of Bushen Qiangji Granules on fibroblast proliferation（5 samples

acid phosphatase staining）

period（3 samples

PI flow cytometry）

and apoptosis rate（3 samples

Annexin V flow cytometry） induced by cell factors was observed.Results Affected by the Bushen Qiangji medicinal serum

the proliferation of fibroblasts in AS induced by cell factors was significantly inhibited（P

<

0.01）.The ratio of G

0

/G

1

stage of fibroblasts was significantly increased（P

<

0.05）.The ratio of S stage of fibroblasts was decreased（P

<

0.01）.And the early and late apoptosis rates of fibroblasts were all increased to different degrees（P

<

0.01）.Conclusion Through inhibiting the effect of cell factors on the proliferation

period

and apoptosis of fibroblasts

Bushen Qiangji Granules can control the further differentiation of fibroblasts to osteoblasts.