益肾蠲痹丸对肾虚胶原诱导性关节炎大鼠踝关节骨质破坏的影响

陶黎; 刘梅洁; 薛欣; 潘静华; 刘红; 李艳; 李玉梅; 肖诚; 鞠大宏; 赵宏艳

doi:10.13288/j.11-2166/r.2018.05.015

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益肾蠲痹丸对肾虚胶原诱导性关节炎大鼠踝关节骨质破坏的影响

实验研究 | 更新时间：2021-11-10

- 益肾蠲痹丸对肾虚胶原诱导性关节炎大鼠踝关节骨质破坏的影响
- Impact of Yishen Juanbi Wan（益肾蠲痹丸） on Ankle Bone Destruction of Kidney Deficiency Collagen-induced Arthritis rats
- 中医杂志 2018年59卷第5期页码：420-426
- 作者机构：
  
  1. 中国中医科学院中医基础理论研究所
  2. 中国中医科学院医学实验中心
  3. 中日友好医院临床医学研究所
- 作者简介：
- 基金信息：
  
  国家自然科学基金（81573845,81373773）;中国中医科学院基础所自主选题项目（YZ-1405）
- DOI：10.13288/j.11-2166/r.2018.05.015
  中图分类号： R285.5
- 纸质出版：2018
- 稿件说明：
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[1]陶黎,刘梅洁,薛欣,潘静华,刘红,李艳,李玉梅,肖诚,鞠大宏,赵宏艳.益肾蠲痹丸对肾虚胶原诱导性关节炎大鼠踝关节骨质破坏的影响[J].中医杂志,2018,59(05):420-426.

TAO Li, LIU Meijie, XUE Xin, et al. Impact of Yishen Juanbi Wan（益肾蠲痹丸） on Ankle Bone Destruction of Kidney Deficiency Collagen-induced Arthritis rats[J]. Journal of Traditional Chinese Medicine2018, 59(5): 420-426.
[1]陶黎,刘梅洁,薛欣,潘静华,刘红,李艳,李玉梅,肖诚,鞠大宏,赵宏艳.益肾蠲痹丸对肾虚胶原诱导性关节炎大鼠踝关节骨质破坏的影响[J].中医杂志,2018,59(05):420-426. DOI： 10.13288/j.11-2166/r.2018.05.015.

TAO Li, LIU Meijie, XUE Xin, et al. Impact of Yishen Juanbi Wan（益肾蠲痹丸） on Ankle Bone Destruction of Kidney Deficiency Collagen-induced Arthritis rats[J]. Journal of Traditional Chinese Medicine2018, 59(5): 420-426. DOI： 10.13288/j.11-2166/r.2018.05.015.

摘要

目的探讨益肾蠲痹丸改善骨质破坏的可能作用机制。方法将106只SD大鼠随机分为空白对照组10只、假手术组10只、肾虚关节炎组10只、肾虚关节炎+西药组10只、肾虚关节炎+中药组11只、关节炎组9只、关节炎+西药组10只、关节炎+中药组10只。肾虚关节炎组、肾虚关节炎+西药组、肾虚关节炎+中药组大鼠采用去卵巢和Ⅱ型胶原免疫诱导建立肾虚关节炎模型

关节炎组、关节炎+西药组、关节炎+中药组大鼠采用Ⅱ型胶原免疫诱导建立关节炎模型。关节炎+中药组和肾虚关节炎+中药组大鼠给予益肾蠲痹丸2.20 g/（kg·d）灌胃。关节炎+西药组和肾虚关节炎+西药组大鼠给予戊酸雌二醇0.092 mg/（kg·d）灌胃。每天1次

连续28天。给药后测定大鼠血清雌二醇（E2）含量

观察踝关节病理损伤情况

检测踝关节破骨细胞数量。结果关节炎组大鼠可见不同程度滑膜细胞增生

滑膜组织充血水肿

关节腔内有剥脱的关节软骨及滑膜组织。肾虚关节炎组大鼠可见骨小梁稀疏变细

间隙增大。关节炎+中药组及肾虚关节炎+中药组大鼠滑膜组织增生及滑膜组织充血水肿明显减轻

血管增生和浸润炎细胞数量及血管翳形成有不同程度减轻。与肾虚关节炎组比较

肾虚关节炎+西药组、肾虚关节炎+中药组大鼠破骨细胞数量减少

E2含量升高（P<0.05或P<0.01）。与关节炎组比较

关节炎+中药组大鼠破骨细胞数量降低

E2含量升高（P<0.05）。与肾虚关节炎+中药组比较

关节炎+中药组大鼠破骨细胞数量降低

E2含量升高（P<0.01）。结论肾虚可加重关节炎引起的骨质破坏

益肾蠲痹丸可以减轻关节炎及肾虚关节炎大鼠关节骨质破坏程度

可能与抑制踝关节局部破骨细胞的数量、升高血清E2含量有关。

Abstract

Objective To explore the possible mechanism of Yishen Juanbi Wan（益肾蠲痹丸） in improving bone destruction. Methods One hundred and six Sprague Dawley（SD） rats were randomized into blank control group（n = 10）

sham operation group（n = 10）

kidney deficiency arthritis group（n = 10）

kidney deficiency arthritis + western medicine group（n = 10）

kidney deficiency arthritis + Chinese medicine group（n = 11）

arthritis group（n = 9）

arthritis + western medicine group（n = 10） and arthritis + Chinese medicine group（n = 10）. Rats in the kidney deficiency arthritis group

the kidney deficiency arthritis + western medicine group and the kidney deficiency arthritis + Chinese medicine group were made kidney deficiency arthritis models by ovariectomy and immune induction of typeⅡ collagen. Rats in the arthritis group

the arthritis + western medicine group and the arthritis +Chinese medicine group were made arthritis models by immune induction of type Ⅱcollagen. Rats in the arthritis +Chinese medicine group and the kidney deficiency arthritis + Chinese medicine group were given Yishen Juanbi Wan2. 20 g/（kg·d） by gavage. Rats in the arthritis + western medicine group and the kidney deficiency arthritis + western medicine group were given estradiol valerate 0. 092 mg/（kg·d） by gavage

once every day for 28 days. After medication

serum estradiol（E2） level was detected. Pathological injury of ankle joint was examined. The number of ankle osteoclasts was detected. Results Different levels of synovial cell proliferation

synovial hyperemia and edema

as well as exfoliated articular cartilage and synovial tissue in articular cavity were found in the arthritis group rats. Bone trabecular thinning and gap increase were found in the kidney deficiency arthritis group rats. Synovial tissue proliferation and synovial hyperemia and edema seemed reduced in the arthritis + Chinese medicine group rats and the kidney deficiency arthritis + Chinese medicine group rats. Vascular proliferative

infiltrating inflammatory cell number and pannus formation decreased in varying degrees. Compared with the kidney deficiency arthritis group

rats’ osteoclasts decreased and E2 increased in the kidney deficiency arthritis + western medicine group and the kidney deficiency arthritis + Chinese medicine group（P < 0. 05 or P < 0. 01）. Compared with the arthritis group

rats’ osteoclasts decreased and E2 increased in the arthritis + Chinese medicine group（P < 0. 05）. Compared with the kidney deficiency arthritis + Chinese medicine group

rats’ osteoclasts decreased and E2 increased in the arthritis + Chinese medicine group（P < 0. 01）. Conclusion Kidney deficiency might exacerbate bone destruction caused by arthritis. Yishen Juanbi Wan might reduce joint bone destruction of arthritis and kidney deficiency arthritis rats

which might relate to inhibiting ankle local osteoclast number and increasing serum E2 level.

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