银屑1号对Trappin-2刺激下肿瘤坏死因子α干预造模的HaCaT细胞分泌NE、Trappin-2的影响

刘靖; 涂绍忠; 何亚男; 陈智斌; 陈梦雅; 查旭山

doi:10.13288/j.11-2166/r.2018.17.014

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银屑1号对Trappin-2刺激下肿瘤坏死因子α干预造模的HaCaT细胞分泌NE、Trappin-2的影响

实验研究 | 更新时间：2021-11-10

- 银屑1号对Trappin-2刺激下肿瘤坏死因子α干预造模的HaCaT细胞分泌NE、Trappin-2的影响
- Effects of Yinxie Yihao Prescription（银屑 1 号） on Neutrophil Elastase and Trappin-2 Secreted by HaCaT Cells Interfered and Modeled by Tumor Necrosis Factor α Stimulated by Trappin-2
- 中医杂志 2018年59卷第17期页码：1498-1502
- 作者机构：
  
  1. 广州中医药大学
  2. 广州中医药大学第一附属医院
  3. 深圳市龙岗区中医院/北京中医药大学深圳医院
- 作者简介：
- 基金信息：
  
  国家自然科学基金（81202699,81573980）
- DOI：10.13288/j.11-2166/r.2018.17.014
  中图分类号： R285.5
- 纸质出版：2018
- 稿件说明：
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[1]刘靖,涂绍忠,何亚男,陈智斌,陈梦雅,查旭山.银屑1号对Trappin-2刺激下肿瘤坏死因子α干预造模的HaCaT细胞分泌NE、Trappin-2的影响[J].中医杂志,2018,59(17):1498-1502.

LIU Jing, TU Shaozhong, HE Ya’nan, et al. Effects of Yinxie Yihao Prescription（银屑 1 号） on Neutrophil Elastase and Trappin-2 Secreted by HaCaT Cells Interfered and Modeled by Tumor Necrosis Factor α Stimulated by Trappin-2[J]. Journal of Traditional Chinese Medicine2018, 59(17): 1498-1502.
[1]刘靖,涂绍忠,何亚男,陈智斌,陈梦雅,查旭山.银屑1号对Trappin-2刺激下肿瘤坏死因子α干预造模的HaCaT细胞分泌NE、Trappin-2的影响[J].中医杂志,2018,59(17):1498-1502. DOI： 10.13288/j.11-2166/r.2018.17.014.

LIU Jing, TU Shaozhong, HE Ya’nan, et al. Effects of Yinxie Yihao Prescription（银屑 1 号） on Neutrophil Elastase and Trappin-2 Secreted by HaCaT Cells Interfered and Modeled by Tumor Necrosis Factor α Stimulated by Trappin-2[J]. Journal of Traditional Chinese Medicine2018, 59(17): 1498-1502. DOI： 10.13288/j.11-2166/r.2018.17.014.

摘要

目的探讨银屑1号治疗银屑病的可能作用机制。方法 24只大鼠随机分为银屑1号组、雷公藤组、空白组

每组8只。银屑1号组大鼠给予银屑1号煎煮浓缩液196 g/（kg·d）灌胃

雷公藤组给予雷公藤多甙片混悬液6 mg/（kg·d）灌胃

空白组给予生理盐水灌胃

均每日2次

每次5 ml

连续5天

制备含药血清。体外培养Ha Ca T细胞

以肿瘤坏死因子α（TNF-α）刺激Ha Ca T细胞建立银屑病细胞模型。实验设置空白组、雷公藤组、抑制剂组、银屑1号组、抑制剂+银屑1号组。空白组、雷公藤组、银屑1号组分别加入基础培养基3.8 ml与相应含药血清

抑制剂组、抑制剂+银屑1号组分别加入空白含药血清和银屑1号含药血清

同时均加入Trappin-2（0.3μg/ml）培养基3.8 ml。体外培养48 h

检测Ha Ca T细胞中中性粒细胞弹性蛋白酶（NE）、Trappin-2含量及NE、Trappin-2 mRNA表达。结果与空白组比较

雷公藤组、抑制剂组、银屑1号组、抑制剂+银屑1号组NE、Trapppin-2含量及mRNA含量明显降低（P<0.05或P<0.01）。与雷公藤组比较

抑制剂组、银屑1号组、抑制剂+银屑1号组NE、Trapppin-2含量及mRNA表达明显升高（P<0.01）。与抑制剂组比较

银屑1号组、抑制剂+银屑1号组NE、Trapppin-2表达明显降低（P<0.05或P<0.01）;银屑1号组NE mRNA表达明显升高（P<0.01）;抑制剂+银屑1号组Trapppin-2mRNA表达降低（P<0.05）。与银屑1号组比较

抑制剂+银屑1号组NE、Trapppin-2含量及mRNA表达明显降低（P<0.05或P<0.01）。结论银屑1号可降低Ha Ca T细胞中NE、Trappin-2含量及基因表达水平

从而减轻炎症反应

可能是其治疗银屑病的作用机制之一。

Abstract

Objective To explore the possible mechanism of Yinxie Yihao Prescription（YXYHP）（银屑 1 号） on psoriasis. Methods A total of 24 rats were randomly divided into YXYHP group

Leigongteng group and blank group

with 8 rats in each group. The rats in YXYHP group were given concentrate decoction of YXYHP 196 g/（kg·d） in gavage. Rats in Leigongteng group were given suspension liquid of Triperygium wilfordii multiglucoside tablet6 mg/（kg·d） in gavage. Rats in the blank group were given normal saline in gavage

twice a day

5 ml each time for continuous 5 days. The serum containing medicine from each group was prepared. The psoriatic cell model was established stimulating HaCaT cell cultivated in vitro with tumor necrosis factor α（TNF-α）. The experiment set blank group

Leigongteng group

inhibitor group

Chinese medicine group

and inhibitor + Chinese medicine group. The blank group

Leigongteng group

and Chinese medicine group were added basal culture medium 3. 8 ml

Trappin-2 culture medium and serum containing Chinese medicine respectively. The inhibitor group

and inhibitor + Chinese medicine group were added Trappin-2（0. 3 μg/ml） culture medium 3. 8 ml and Trappin-2 culture medium serum containing related medicine. After culturing in vitro for 48 h

the neutrophil elastase（NE）

Trapinin-2 and expression of NE

Trappin-2 mRNA in HaCaT cells were detected. Results Compared with the control group

the contents of NE

Trappin-2 and mRNA expression in Leigongteng group

inhibitor group

Chinese medicine group

and inhibitor +Chinese medicine group were significantly decreased（P < 0. 05 or P < 0. 01）. Compared with Leigongteng group

the contents of NE

Trappin-2 and mRNA expression in inhibitor group

Chinese medicine group

and inhibitor + Chinese medicine group were significantly increased（P < 0. 01）. Compared with the inhibitor group

the expression of NE and Trappin-2 in the Chinese medicine group

inhibitor + Chinese medicine group was significantly decreased（P <0. 05 or P < 0. 01）. The NE and mRNA expression in the Chinese medicine group was significantly increased（P <0. 01）. The expression of Trappin-2 mRNA was decreased in inhibitor + Chinese medicine group（P < 0. 05）. Compared with the Chinese medicine group

NE and Trappin-2 contents and mRNA expression in the inhibitor + Chinese medicine group were significantly decreased（P < 0. 05 or P < 0. 01）. Conclusion YXYHP can reduce the contents of NE

Trappin-2 and the gene expression level in HaCaT cells

thus reducing inflammatory response

which may be one of the action mechanisms in treating psoriasis.

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