左归降糖解郁方对糖尿病并发抑郁症大鼠海马趋化因子CX3C受体1和炎症因子的影响

杨蕙; 王宇红; 孟盼; 杜青; 柳卓; 刘检; 杨琴; 凌佳

doi:10.13288/j.11-2166/r.2018.20.015

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左归降糖解郁方对糖尿病并发抑郁症大鼠海马趋化因子CX3C受体1和炎症因子的影响

实验研究 | 更新时间：2021-11-10

- 左归降糖解郁方对糖尿病并发抑郁症大鼠海马趋化因子CX3C受体1和炎症因子的影响
- Effects of Zuogui Jiangtang Jieyu Decoction （左归姜汤解郁方） on Hippocampal Chemokine CX3C Receptor 1 and Neuroinflammatory Factors in Hippocampus of Diabetic Rats with Depression
- 中医杂志 2018年59卷第20期页码：1783-1787
- 作者机构：
  
  1. 湖南中医药大学第一附属医院
  2. 湖南省中药粉体与创新药物省部共建国家重点实验室培育基地
  3. 湖南省中医药研究院
- 作者简介：
- 基金信息：
  
  湖南省教育厅科研项目（17B201）;国家自然科学基金（81403379）
- DOI：10.13288/j.11-2166/r.2018.20.015
  中图分类号： R285.5
- 纸质出版：2018
- 稿件说明：
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[1]杨蕙,王宇红,孟盼,杜青,柳卓,刘检,杨琴,凌佳.左归降糖解郁方对糖尿病并发抑郁症大鼠海马趋化因子CX3C受体1和炎症因子的影响[J].中医杂志,2018,59(20):1783-1787.

YANG Hui, WANG Yuhong, MENG Pan, et al. Effects of Zuogui Jiangtang Jieyu Decoction （左归姜汤解郁方） on Hippocampal Chemokine CX3C Receptor 1 and Neuroinflammatory Factors in Hippocampus of Diabetic Rats with Depression[J]. Journal of Traditional Chinese Medicine2018, 59(20): 1783-1787.
[1]杨蕙,王宇红,孟盼,杜青,柳卓,刘检,杨琴,凌佳.左归降糖解郁方对糖尿病并发抑郁症大鼠海马趋化因子CX3C受体1和炎症因子的影响[J].中医杂志,2018,59(20):1783-1787. DOI： 10.13288/j.11-2166/r.2018.20.015.

YANG Hui, WANG Yuhong, MENG Pan, et al. Effects of Zuogui Jiangtang Jieyu Decoction （左归姜汤解郁方） on Hippocampal Chemokine CX3C Receptor 1 and Neuroinflammatory Factors in Hippocampus of Diabetic Rats with Depression[J]. Journal of Traditional Chinese Medicine2018, 59(20): 1783-1787. DOI： 10.13288/j.11-2166/r.2018.20.015.

摘要

目的探讨左归降糖解郁方治疗糖尿病并发抑郁症的可能作用机制。方法 40只SD大鼠随机分为正常组

模型组

西药组及中药高、低剂量组

每组8只。除正常组外

其余各组通过高脂乳剂灌胃、尾静脉注射链脲佐菌素和慢性应激建立糖尿病并发抑郁症模型。应激造模的同时西药组给予二甲双胍0.18 g/（kg·d）+氟西汀1.8 mg/（kg·d）灌胃;中药高、低剂量组分别给予左归降糖解郁方20.53、10.26 g/（kg·d）灌胃

正常组和模型组给予等体积蒸馏水灌胃

均每日1次。灌胃28天后

检测大鼠海马中炎症因子白细胞介素1β（IL-1β）和肿瘤坏死因子α（TNF-α）的含量

小胶质细胞的标记物离子钙接头蛋白分子1（Iba-1）、趋化因子CX3C受体1（CX3CR1）、核转录因子κB（NF-κB）及磷酸化核转录因子κB（p NF-κB）蛋白表达。结果与正常组比较

模型组大鼠海马IL-1β、TNF-α表达及其小胶质细胞Iba-1、CX3CR1、p NF-κB表达均显著升高（P<0.01）。与模型组比较

西药组及中药高剂量组大鼠海马IL-1β和TNF-α表达及其小胶质细胞Iba-1、CX3CR1、p NF-κB表达均显著下降

中药低剂量组IL-1β、TNF-α、CX3CR1和p NF-κB表达亦下降（P<0.05或P<0.01）。与中药高剂量组比较

中药低剂量组CX3CR1及p NF-κB表达显著升高（P<0.05或P<0.01）。结论左归降糖解郁方可显著降低糖尿病并发抑郁症大鼠的海马炎症水平

该作用可能与其抑制小胶质细胞激活并下调CX3CR1和NF-κB表达有关。

Abstract

Objective To explore the possible mechanism of Zuogui Jiangtang Jieyu Decoction（左归姜汤解郁方） in treating diabetic rats with depression. Methods A total of 40 SD rats were randomly divided into normal group

model group

western medicine group and traditional Chinese medicine（ TCM） high and low dose groups

with 8 rats in each group. Except for the normal group

rats were given administration of high fat emulsion

injection of streptozotocin（ STZ） via coccygeal vein

and chronic stress to establish rat models of diabetes complicated with depression. During chronic stress modeling

the western medicine group was administrated with metformin 0. 18 g/（ kg·d） and fluoxetine 1. 8 mg/（ kg·d）

and the TCM high and low dose groups were respectively given Zuogui Jiangtang Jieyu Decoction 20. 53

10. 26 g/（ kg·d）. The normal group and the model group were given an equal volume of distilled water once a day. After 28 days

the levels of interleukin 1β（ IL-1β） and tumor necrosis factor-α（ TNF-α） in hippocampus were detected

and the expression of ionized calcium-binding adapter molecule 1（ Iba-1）

chemotactic factor CX3 C receptor 1（ CX3 CR1）

nuclear transcription factor-κB（ NF-κB） and phosphorylated nuclear transcription factors-κB（ p NF-κB） proteins expressions in rats were determined. Results Compared with the normal group

the expression of IL-1β and TNF-α in hippocampus were significantly increased（ P < 0. 01）

the expression of Iba-1

CX3 CR1 and p NF-κB in the model group were significantly increased（ P < 0. 01）. Compared with the model group

the expression of IL-1β and TNF-α and the expression of Iba-1

CX3 CR1 and p NF-κB in western medicine group TCM high dose group was decreased significantly

and the expression of IL-1β

TNF-α; CX3 CR1 and p NF-κB in TCM low dose group was decreased（ P < 0. 05 or P < 0. 01）. Compared with TCM high dose group

CX3 CR1 and p NF-κB expressions in TCM low dose group increased significantly（ P < 0. 05 or P < 0. 01）. Conclusion Zuogui Jiangtang Jieyu Decoction can significantly reduce the level of hippocampal inflammation factors in diabetic rats with depression

which may be related to the function of microglial activation inhibition and down-regulation the expression of CX3 CR1 and NF-κB.

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