Objective To explore the possible mechanism of Shugan Jianpi Huoxue Decoction（ 疏肝健脾活血方）in treating liver sinus capillarification in liver fibrosis. Methods The hepatic stellate cells（ HSC） and liver sinusoidal endothelial cell（ LSEC） from normal rats

LSEC from liver fibrosis model rats were separated. The vascular endothelial growth factor（ VEGF） overexpressed HSC was obtained by virus transfection. The cells were divided into six groups: a) normal HSC group

cultured HSC by blank serum; b) normal group: co-culture normal HSC and normal LSEC by blank serum; c) model group: co-culture normal HSC and model LSEC by blank serum; d) overexpression group: co-culture VEGF overexpressed HSC and model LSEC by blank serum; e) model with herbs group:co-culture normal HSC and model LSEC by 10% drug containing serum; f) overexpression with herbs group: coculture VEGF overexpressed HSC and model LSEC by 10% drug containing serum. Western Blot method was used to detect the expression of CD31 and von Willebrand factor（ vWF） in LSEC and the expression of collagen Ⅳ

VEGF and vascular endothelial growth factor receptor 2（ VEGFR-2） in HSC. The expression of VEGF

vascular endothelial growth factor receptor 1（ VEGFR-1） and VEGFR-2 mRNA in HSC was detected by PCR. Results Compared with normal group

the expression of CD31 and vWF in model group and overexpression group was increased（ P < 0. 01）

and the expression of CD31 and v WF in the model with herbs group was decreased when compared with model group（ P < 0. 01）. Compared with normal HSC group and normal group

the expression of collagen Ⅳ and VEGF in model group and overexpression group was increased（ P < 0. 01）

and the expression of VEGF and VEGFR-2 in the model with herbs group was decreased when compared with model group（ P < 0. 05）. Compared with normal HSC group and normal group

the expression of VEGF mRNA in model group and overexpression group was increased（ P < 0. 01）.The expression of VEGF mRNA in model with herbs group was decreased than that in model group（ P < 0. 05）.Conclusion Shugan Jianpi Huoxue Decoction reverse the capillary vascularization of hepatic sinus possibly by downregulating the expression of VEGF and VEGFR-2

inhibiting the signal pathway of VEGF and restoring the window structure of LSEC.